…who joins the group for his MSc thesis. In collaboration with the group of Stan Brouns (now Delft, previously WUR) he will enzymatically modify DNA to allow the labelling with fluorescent dyes and then image the labelled DNA in vivo and in vitro using super-resolution microscopy.
…who joins the group for his PhD thesis. In collaboration with the groups of John van Duynhoven (Unilever and Laboratory of Biophysics), Aldrik Velders (Laboratory of Bionanotechnology) and Atze Jan van der Groot (Food Process Engineering) he will study anisotropic food structures using single-particle diffusometry.
We just got the very first version from the workshop to test the general design layout. Looks promising. The cube went back to drill and tap a few more holes and to receive a black anodisation.
…who joins the group for his PhD thesis. In collaboration with the group of Dolf Weijers (Laboratory of Biochemistry) he will study the Auxin Response Factor (ARF), which is an important transcription factor regulating cell growth and differentiation in plants, using a variety of spectroscopic methods.
Good news! Our proposal “Probing submicron anisotropic food structures using single-nanoparticle diffusometry” has been granted by the Graduate School VLAG [link] allowing us to hire a new PhD student.
In close collaboration with Prof. John van Duyhhoven (Laboratory of Biophysics, Wageningen), Prof. Aldrik Velders (Laboratory of BioNanoTechnology, Wageningen) and Prof. Atze Jan van der Goot (Laboratory of Food Process Engineering, Wageningen), we aim to develop and utilise fluorescence-based assays to study scale-dependent structural anisotropy in biopolymeric gels and food materials. Building on our broad expertise on single-particle tracking and imaging, we hope to establish single-molecule techniques as a new toolkit in food-related research.
Update: Follow the link or Twitter (#miCube) for the lastest information.
Fluorescence microscopy is an extremely powerful and versatile technique contributing to many areas of the life sciences. Especially variants featuring the ability to monitor single-molecule fluorescence, however, require sophisticated instrumentation that is either very expensive when bought commercially (>> 100 kEuro) or demands extensive expertise in optics and engineering.
Here we present an open and modular hardware framework aiming for
- cost effectiveness: build your own starting at 20k Euro (~100 kEuro for state of the art capabilities)
- modularity: all parts can be accessed and replaced by the user
- simplicity: set up the microscope in a few hours without prior knowledge
- customizability: confocal or widefield/TIRF microscopy,…
- openness: part lists and drawings will be made available
- stability and throughput: minimizing drift and utilise well plate scanners
Interested? Drop me a line. We are currently working together with several academic labs to bring their ideas to life and develop the miCube concept further.
For a similar concept, please also visit http://wosmic.org/.
Good news! Our proposal “Illuminating plant hormone responses at the single-molecule level” has been granted by the Graduate School Experimental Plant Sciences [link].
In close collaboration with Prof. Dolf Weijers from the Laboratory of Biochemistry (Wageningen), we aim to develop and utilise new fluorescence-based assays to quantify inter- and intra-molecular interactions in the auxin mediated signalling pathway. By applying techniques such as single-molecule Förster resonance energy transfer (smFRET), we will characterise the binding kinetics of various ARF-DBD proteins to DNA whilst monitoring structural changes of the DNA and the associated proteins.
…who joined our lab for a six-month internship. Timo is studying Medical Engineering at the University zu Luebeck, Germany and he is going to work on the nanofluidics project.
…who both joined our lab for their BSc theses. Stan and Rebecca are both studying Molecular Life Sciences in Wageningen. Rebecca will work on detecting DNA polymerisation with fluorescently labelled DNA polymerases and Stan will update our software framework for super-resolution microscopy and single particle tracking.
…who both joined our lab for an internship (Lonneke) and a BSc thesis (Ebru). Lonneke, who studies Chemistry at the Hogeschool Leiden, is going to work on single-particle tracking in food-related biopolymers. Ebru, who studies Biotechnologie at Hogeschool Van Hall Larenstein in Leeuwarden, will support Carel and develop new dynamic DNA binding assays.