News: Welcome to Jan Groen…

…who joins the group for his MSc thesis. In collaboration with the group of Stan Brouns (now Delft, previously WUR) he will enzymatically modify DNA to allow the labelling with fluorescent dyes and then image the labelled DNA in vivo and in vitro using super-resolution microscopy.

News: VLAG proposal granted

Good news! Our proposal “Probing submicron anisotropic food structures using single-nanoparticle diffusometry” has been granted by the Graduate School VLAG [link] allowing us to hire a new PhD student.

In close collaboration with Prof. John van Duyhhoven (Laboratory of Biophysics, Wageningen), Prof. Aldrik Velders (Laboratory of BioNanoTechnology, Wageningen) and Prof. Atze Jan van der Goot (Laboratory of Food Process Engineering, Wageningen), we aim to develop and utilise fluorescence-based assays to study scale-dependent structural anisotropy in biopolymeric gels and food materials. Building on our broad expertise on single-particle tracking and imaging, we hope to establish single-molecule techniques as a new toolkit in food-related research.

Introducing miCube V0.1: Rethinking modular fluorescence microscopy

Update: Follow the link or Twitter (#miCube) for the lastest information.

Fluorescence microscopy is an extremely powerful and versatile technique contributing to many areas of the life sciences. Especially variants featuring the ability to monitor single-molecule fluorescence, however, require sophisticated instrumentation that is either very expensive when bought commercially (>> 100 kEuro) or demands extensive expertise in optics and engineering.

Here we present an open and modular hardware framework aiming for

  • cost effectiveness: build your own starting at 20k Euro (~100 kEuro for state of the art capabilities)
  • modularity: all parts can be accessed and replaced by the user
  • simplicity: set up the microscope in a few hours without prior knowledge
  • customizability: confocal or widefield/TIRF microscopy,…
  • openness: part lists and drawings will be made available
  • stability and throughput: minimizing drift and utilise well plate scanners

Interested? Drop me a line. We are currently working together with several academic labs to bring their ideas to life and develop the miCube concept further.

CompleteSetup

For a similar concept, please also visit http://wosmic.org/.

News: EPS proposal granted

Good news! Our proposal “Illuminating plant hormone responses at the single-molecule level” has been granted by the Graduate School Experimental Plant Sciences [link].

In close collaboration with Prof. Dolf Weijers from the Laboratory of Biochemistry (Wageningen), we aim to develop and utilise new fluorescence-based assays to quantify inter- and intra-molecular interactions in the auxin mediated signalling pathway. By applying techniques such as single-molecule Förster resonance energy transfer (smFRET), we will characterise the binding kinetics of various ARF-DBD proteins to DNA whilst monitoring structural changes of the DNA and the associated proteins.

News: Welcome to Timo Wenzel…

…who joined our lab for a six-month internship. Timo is studying Medical Engineering at the University zu Luebeck, Germany and he is going to work on the nanofluidics project.

News: Welcome to Ebru Acun and Lonneke Zuidgeest…

…who both joined our lab for an internship (Lonneke) and a BSc thesis (Ebru). Lonneke, who studies Chemistry at the Hogeschool Leiden, is going to work on single-particle tracking  in food-related biopolymers. Ebru, who studies Biotechnologie at Hogeschool Van Hall Larenstein in Leeuwarden, will support Carel and develop new dynamic DNA binding assays.