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- Pre-print: An open microscopy framework suited for tracking dCas9 in live bacteria
- Published: Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
- News: The miCube moved to Github
- Pre-print: DNA polymerase β fingers movement revealed by single-molecule FRET suggests a partially closed conformation as a fidelity checkpoint
- News: Welcome to Meike Kronenberg and farewell to Carel Fijen
Category Archives: miCube
Our open (single-molecule) microscopy project, the #miCube, is now on Github [link]! The page shows a detailed and updated overview of the components, some information on phasor-based SMLM, and many links to similar open hardware projects.
Here the specs: 50k frames, 850k localisations, 6 min for data analysis. Many thanks to Sander Baas, Koen Martens and Arjen Bader, with cells provided by Gert-Jan Bakker and Ben Joosten (both Radboudumc, Nijmegen).
Hurrah, we finalised the design of our miCube (V0.1)! Details can be found [here] and include part numbers, CAD-drawings and STL files for CNC machining/milling or 3D printing published under a Creative Common license. Big shout out to Sander Baas … Continue reading
We finally managed to perform some test experiments and it is looking very promising! We tracked small fluorescent quantum dots (< 5 nm diameter) diffusing in water (20% glycerol) and we were happy to learn that even for such a … Continue reading
We just got the very first version from the workshop to test the general design layout. Looks promising. The cube went back to drill and tap a few more holes and to receive a black anodisation.
Update: Follow the link or Twitter (#miCube) for the lastest information. Fluorescence microscopy is an extremely powerful and versatile technique contributing to many areas of the life sciences. Especially variants featuring the ability to monitor single-molecule fluorescence, however, require sophisticated … Continue reading