News: The miCube moved to Github

Our open (single-molecule) microscopy project, the #miCube, is now on Github [link]!  The page shows a detailed and updated overview of the components, some information on phasor-based SMLM, and many links to similar open hardware projects.

 

miCube V0.1: first tests

We finally managed to perform some test experiments and it is looking very promising! We tracked small fluorescent quantum dots (< 5 nm diameter) diffusing in water (20% glycerol) and we were happy to learn that even for such a demanding application new sCMOS-based cameras are doing just fine. Here a picture of the current assembly. More soon!

micubeassembled

Introducing miCube V0.1: Rethinking modular fluorescence microscopy

Update: Follow the link or Twitter (#miCube) for the lastest information.

Fluorescence microscopy is an extremely powerful and versatile technique contributing to many areas of the life sciences. Especially variants featuring the ability to monitor single-molecule fluorescence, however, require sophisticated instrumentation that is either very expensive when bought commercially (>> 100 kEuro) or demands extensive expertise in optics and engineering.

Here we present an open and modular hardware framework aiming for

  • cost effectiveness: build your own starting at 20k Euro (~100 kEuro for state of the art capabilities)
  • modularity: all parts can be accessed and replaced by the user
  • simplicity: set up the microscope in a few hours without prior knowledge
  • customizability: confocal or widefield/TIRF microscopy,…
  • openness: part lists and drawings will be made available
  • stability and throughput: minimizing drift and utilise well plate scanners

Interested? Drop me a line. We are currently working together with several academic labs to bring their ideas to life and develop the miCube concept further.

CompleteSetup

For a similar concept, please also visit http://wosmic.org/.