Category Archives: open access

Pre-print: Simple nanofluidic devices for high-throughput, non-equilibrium studies at the single-molecule level

C. Fijen, M. Fontana, S.G. Lemay, K. Mathwig, J. Hohlbein, bioRxiv, 2017, [link] Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples. Probing chemical and biological interactions and reactions with high throughput and … Continue reading

Posted in open access, pre-print

Pre-print: Phasor based single-molecule localization microscopy in 3D (pSMLM-3D): an algorithm for MHz localization rates using standard CPUs

K.J.A. Martens, A.N. Bader, S. Baas, B. Rieger, J. Hohlbein, bioRxiv, 2017, [link] We present a fast and model-free 2D and 3D single-molecule localization algorithm that allows more than 3 million localizations per second on a standard multi-core CPU with localization … Continue reading

Posted in open access, pre-print

News: (Update) miCube: Rethinking modular fluorescence microscopy

Hurrah, we finalised the design of our miCube (V0.1)! Details can be found [here] and include part numbers, CAD-drawings and STL files for CNC machining/milling or 3D printing published under a Creative Common license. Big shout out to Sander Baas … Continue reading

Posted in miCube, news, open access

Publication: Visualization of BRI1 and SERK3/BAK1 Nanoclusters in Arabidopsis Roots

J. Hohlbein and A.N. Kapanidis, Methods in Enzymology: Single-molecule Enzymology Part A & B, published online, 2016, [link] Monitoring conformational changes in DNA polymerases using single-molecule Förster resonance energy transfer (smFRET) has provided new tools for studying fidelity-related mechanisms that promote the rejection of incorrect nucleotides before DNA synthesis. In addition to the previously known open and the […]

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Posted in open access, published

News: miCube V0.1 prototyping

We just got the very first version from the workshop to test the general design layout. Looks promising. The cube went back to drill and tap a few more holes and to receive a black anodisation.

Posted in miCube, news, open access

Publication: Fluorescence resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers

E. Ploetz, E. Lerner, F. Husada, M. Roelfs, S. Chung, J. Hohlbein, S. Weiss, T. Cordes, Scientific Reports, 6, 33257 , 2016, [link] Advanced microscopy methods allow obtaining information on (dynamic) conformational changes in biomolecules via measuring a single molecular distance … Continue reading

Posted in open access, published

Introducing miCube V0.1: Rethinking modular fluorescence microscopy

Update: Follow the link or Twitter (#miCube) for the lastest information. Fluorescence microscopy is an extremely powerful and versatile technique contributing to many areas of the life sciences. Especially variants featuring the ability to monitor single-molecule fluorescence, however, require sophisticated … Continue reading

Posted in miCube, news, open access