Author Archives: JH

Pre-print: Simple nanofluidic devices for high-throughput, non-equilibrium studies at the single-molecule level

C. Fijen, M. Fontana, S.G. Lemay, K. Mathwig, J. Hohlbein, bioRxiv, 2017, [link] Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples. Probing chemical and biological interactions and reactions with high throughput and … Continue reading

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Pre-print: Phasor based single-molecule localization microscopy in 3D (pSMLM-3D): an algorithm for MHz localization rates using standard CPUs

K.J.A. Martens, A.N. Bader, S. Baas, B. Rieger, J. Hohlbein, bioRxiv, 2017, [link] We present a fast and model-free 2D and 3D single-molecule localization algorithm that allows more than 3 million localizations per second on a standard multi-core CPU with localization … Continue reading

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News: Welcome to Sam van Beljouw…

…who joins the group for his MSc thesis. In collaboration with Dr. Klaus Mathwig (Pharmacy, Groningen), Dr. Peter van Baarlen (Host-Microbe Interactions, Wageningen) and Simon van der Els (NIZO food research and HMI, Wageningen), he will work towards monitoring bacterial conjugation … Continue reading

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Proceeding: A Nanofluidic Mixing Device for High-Throughput Fluorescence Sensing of Single Molecules

K. Mathwig, C. Fijen, M. Fontana, S. G. Lemay and J. Hohlbein, Procedia Technology, 27, 141,  2017, [link] We introduce a nanofluidicmixing device entirely fabricated in glass for the fluorescence detection of single molecules. The design consists of a nanochannel T-junction … Continue reading

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News: CHIPP proposal awarded

Good news! The proposal “Lipid Oxidation Control in Food Emulsions Enabled by Natural Strategies” submitted by Harry Gruppen (WUR) has been awarded a CHIPP grant by The Innovation Fund for Chemistry [link]. Our lab will head the work package titled “Localising oxidation: interfaces and … Continue reading

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miCube: 3D super-resolution microscopy

Here the specs: 50k frames, 850k localisations, 6 min for data analysis. Many thanks to Sander Baas, Koen Martens and Arjen Bader, with cells provided by Gert-Jan Bakker and Ben Joosten (both Radboudumc, Nijmegen).

Posted in miCube

News: IPM proposal granted

Good news! Our proposal “Towards visualizing gene transfer at the single cell level using microfluidic devices” has been granted by the Innovation Program Microbiology, an initiative from the Laboratory of Microbiology at Wageningen University and Research. In collaboration with Dr. Klaus Mathwig … Continue reading

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