Author Archives: JH

Pre-print: Direct visualization of native CRISPR target search in live bacteria reveals Cascade DNA surveillance mechanism

J.N.A. Vink, K.J.A. Martens, M. Vlot, R.E. McKenzie, C. Almendros, B. Estrada Bonilla, D.J.W. Brocken, J. Hohlbein, S.J.J. Brouns, bioRxiv, 2019, [link] CRISPR-Cas systems encode RNA-guided surveillance complexes to find and cleave invading DNA elements. While it is thought that invaders are neutralized … Continue reading

Posted in open access, pre-print

Published: Evaluating single-particle tracking by photo-activation localization microscopy (sptPALM) in Lactococcus lactis

S.P.B. van Beljouw, S. van der Els, K.J. A. Martens, M. Kleerebezem, P.A. Bron, J. Hohlbein, Physical Biology, 16, 035001, 2019, [link] Lactic acid bacteria (LAB) are frequently used in food fermentation and are invaluable for the taste and nutritional value … Continue reading

Posted in published

News: Welcome to Suyeon Yang and George Vogelaar

Suyeon joined the group in November 2018 for her PhD thesis. She will study lipid oxidation at oil/water interfaces using super-resolution microscopy. George just started his MSc thesis to study diffusion of dCas9 in Lactococcus lactis using single-particle tracking PALM.

Posted in news

Published: High-throughput, non-equilibrium studies of single biomolecules using glass made nanofluidic devices

M. Fontana, C. Fijen, S. G. Lemay, K. Mathwig and J. Hohlbein, Lab on a Chip, 19, 79, 2019. [link] Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples. However, probing biomolecular interactions and reactions … Continue reading

Posted in published

Pre-print: An open microscopy framework suited for tracking dCas9 in live bacteria

K.J.A. Martens, S. van Beljouw, S. van der Els, S. Baas, J.N.A. Vink, S.J.J. Brouns, P. van Baarlen, M. Kleerebezem, J. Hohlbein, bioRxiv, 2018 [link] Super-resolution microscopy is frequently employed in the life sciences, but the number of freely accessible … Continue reading

Posted in open access, pre-print

Published: Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study

B. Hellenkamp, S. Schmid, O. Doroshenko, O. Opanasyuk, R. Kühnemuth, S. Rezaei Adariani, B. Ambrose, M. Aznauryan, A. Barth, V. Birkedal, M.E. Bowen, H. Chen, T. Cordes, T. Eilert, C. Fijen, C. Gebhardt, M. Götz, G. Gouridis, E. Gratton, T. … Continue reading

Posted in accepted, open access, pre-print

News: The miCube moved to Github

Our open (single-molecule) microscopy project, the #miCube, is now on Github [link]!  The page shows a detailed and updated overview of the components, some information on phasor-based SMLM, and many links to similar open hardware projects.  

Posted in miCube, news