Publications

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(026) A Nanofluidic Mixing Device for High-Throughput Fluorescence Sensing of Single Molecules, K. Mathwig, C. Fijen, M. Fontana, S. G. Lemay and J. Hohlbein, Procedia Technology, 27, 141 2017, [link]

(025) A single-molecule FRET sensor for monitoring DNA synthesis in real time, C. Fijen, A. Montón Silva, A. Hochkoeppler and J. Hohlbein, Physical Chemistry Chemical Physics, 19, 4222-4230, 2017, [link]

(024) Visualization of BRI1 and SERK3/BAK1 Nanoclusters in Arabidopsis Roots, S.J. Hutten, D.S. Hamers, M.A. an den Toorn, W. van Esse, A. Nolles, C.A. Bücherl, S.C. de Vries, J. Hohlbein, J.W. Borst, PLoS ONE 12(1): e0169905, 2017, [link]

(023) Probing the conformational landscape of DNA polymerases using diffusion-based single-molecule FRET, J. Hohlbein and A.N. Kapanidis, Methods in Enzymology: Single-molecule enzymology Part A & B, 581, 353-378, 2016, [link]

(022) Fluorescence resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers, E. Ploetz, E. Lerner, F. Husada, M. Roelfs, S. Chung, J. Hohlbein, S. Weiss, T. Cordes, Scientific Reports, 6, 33257 , 2016, [link]

(021) A Quantitative Theoretical Framework For PIFE-FRET, E. Lerner, E. Ploetz, J. Hohlbein, T. Cordes, S. Weiss, The Journal of Physical Chemistry B, 120, 6401–6410, 2016, [link]

(020) Single Molecule 3D Orientation in Time and Space: A 6D Dynamic Study on Fluorescently Labeled Lipid Membranes, R. Börner, N. Ehrlich, J. Hohlbein, C.G. Hübner, Journal of Fluorescence, 26, 963-975,  2016 [link]

(019) Complex coacervate core micelles with spectroscopic labels for diffusometric probing of biopolymer networks, N. Bourouina, D. de Kort, F. Hoeben, H. Janssen, H. Van As, J. Hohlbein, J. van Duynhoven, J.M. Kleijn, Langmuir, 31, 12635-12643, 2015, [link]

(018) New technologies for DNA analysis – a review of the READNA Project, S. McGinn, D. Bauer, T. Brefort, L. Dong, A. El-Sagheer, A. Elsharawy, G. Evans, E. Falk-Sörqvist, M. Forster, S. Fredriksson, P. Freeman, C. Freitag, J. Fritzsche, S. Gibson, M. Gullberg, M. Gut, S. Heath, I. Heath-Brun, A.J. Heron, J. Hohlbein, R. Ke, O. Lancaster, L. Le Reste, G. Maglia, R. Marie, F. Mauger, F. Mertes, M. Mignardi, L. Moens, J. Oostmeijer, R. Out, J. Nyvold Pedersen, F. Persson, V. Picaud, D. Rotem, N. Schracke, J. Sengenes, P.F. Stähler, B. Stade, D. Stoddart, X. Teng, C.D. Veal, N. Zahra, H. Bayley, M. Beier, T. Brown, C. Dekker, B. Ekström, H. Flyvbjerg, A. Franke, S. Guenther, A.N. Kapanidis, J. Kaye, A. Kristensen, H. Lehrach, J. Mangion, S. Sauer, E. Schyns, J. Tost, J.M.L.M. van Helvoort, P.J. van der Zaag, J. O. Tegenfeldt, A.J. Brookes, K.Mir, M. Nilsson, S. Willcocks, I.G. Gut, New Biotechnology, 33, 310-330, 2016, [link]

(017) Camera-based single-molecule FRET detection with improved time resolution, S. Farooq and J. Hohlbein, Physical Chemistry Chemical Physics, 17, 27862, 2015, [link], open access

(016) Real-time single-molecule studies of the motions of DNA polymerase fingers illuminate DNA synthesis mechanisms, G.W. Evans, J. Hohlbein, T. Craggs, L. Aigrain and A.N. Kapanidis, Nucleic Acids Research, 43, 5998-6008, 2015, [link], open access

(015) Studying DNA-protein interactions with single-molecule Förster resonance energy transfer, S. Farooq, C. Fijen, J. Hohlbein, Protoplasma, SPECIAL ISSUE: NEW/EMERGING TECHNIQUES IN BIOLOGICAL MICROSCOPY, 251317-332, 2014 [link]

(014) Alternating-laser excitation: single-molecule FRET and beyond, J. Hohlbein, T.D. Craggs, T. Cordes, Chemical Society Reviews, 43, 1156-1171, 2014, [link]

(013)  A Novel Parallel Nanomixer for High-Throughput Single-Molecule Fluorescence Detection, K. Mathwig, S. Schlautmann, S. G. Lemay, J. Hohlbein,, Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Science, Freiburg, Germany, Oct. 27 – 31 (2013) 1385,  [link]

(012) Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion, J. Hohlbein, L. Aigrain, T.D. Craggs, O. Bermek, O. Potapova, P. Shoolizadeh, N.D.F. Grindley, C.M. Joyce, A.N. Kapanidis, Nature Communications, 4,  2131, 2013, [link]

(011) Characterization of dark quencher chromophores as non-fluorescent acceptors for single-molecule FRET, L. Le Reste, J. Hohlbein, K. Gryte, A.N. Kapanidis, Biophysical Journal, 102, 2658-2668, 2012, [link]

(010) Identifying molecular dynamics in single-molecule FRET experiments with Burst Variance Analysis, J.P. Torella, S.J. Holden, Y. Santoso, J. Hohlbein, A.N. Kapanidis, Biophysical Journal, 100, 1568-1577, 2011, [link]

(009) Defining the limits of single-molecule FRET resolution in TIRF microscopy, S.J. Holden, S. Uphoff, J. Hohlbein, D. Yadin, L. Le Reste, O.J. Britton, A.N. Kapanidis, Biophysical Journal, 99, Issue 9, 2011, 3102-3111 [link]

(008) Surfing on a new wave of single-molecule methods, J. Hohlbein, K. Gryte, M. Heilemann, A.N. Kapanidis, Physical Biology, 7, 031001, 2010, [link]

(007) Conformational transitions in DNA polymerase I revealed by single-molecule FRET, Y. Santoso, C.M. Joyce, O. Potapova, L. Le Reste, J. Hohlbein, J.P. Torella, N.D.F. Grindley, and A.N. Kapanidis, Proceedings of the National Academy of Sciences of the USA, 107, 715, 2010, [link]

(006) Single-Molecule FRET: Methods and Biological Applications in Handbook of Single-molecule Biophysics, L.C. Hwang, J. Hohlbein, S.J. Holden, and A.N. Kapanidis. Springer-Verlag New York Inc., edit. P. Hinterdorfer and A. Van Oijen, 2009

(005) Three-dimensional orientation determination of the emission dipoles of single molecules: The shot noise limit, J. Hohlbein, and C.G. Huebner, Journal of Chemical Physics, 129, 094703, 2008, [link]

(004) Confined diffusion in ordered nanoporous alumina membranes, J. Hohlbein, M. Steinhart, C. Schiene-Fischer, A. Benda, M. Hof and C.G. Hübner, Small, 3, 380, 2007, [link]

(003) A simple scheme for rapid three-dimensional orientation determination of the emission dipole of single molecules, J. Hohlbein and C.G. Hübner, Applied Physics Letters, 86, 121104, 2005, [link]

(002) Recurrence and photon statistics in fluorescence fluctuation spectroscopy, G. Zumofen, J. Hohlbein, and C.G. Hübner, Physical Review Letters, 93, 260601, 2004, [link]

(001) In-situ optical characterisation of porous alumina, J. Hohlbein, U. Rehn, and R. B. Wehrspohn, Physica Status Solidi (a), 204, 803, 2004, [link]