Good news! Our proposal “Illuminating plant hormone responses at the single-molecule level” has been granted by the Graduate School Experimental Plant Sciences [link].
In close collaboration with Prof. Dolf Weijers from the Laboratory of Biochemistry (Wageningen), we aim to develop and utilise new fluorescence-based assays to quantify inter- and intra-molecular interactions in the auxin mediated signalling pathway. By applying techniques such as single-molecule Förster resonance energy transfer (smFRET), we will characterise the binding kinetics of various ARF-DBD proteins to DNA whilst monitoring structural changes of the DNA and the associated proteins.
Hohlbein lab - Wageningen 