Great to see so many people joining the group. Abbas started his PhD thesis on super-resolution based localisation of biomolecules at food-related interfaces. Ben (BSc thesis, BIP) started illuminating bacteria using the PAINT technique. Vincent (MSc thesis, BIP) supports Koen in his quest to monitor particle diffusion in meat replacers. Last but not least, Sven (BSc thesis with BIC & BIP) will study the influence of control elements to the transcription factor binding.
Some PAINT data by Ben
Suyeon joined the group in November 2018 for her PhD thesis. She will study lipid oxidation at oil/water interfaces using super-resolution microscopy. George just started his MSc thesis to study diffusion of dCas9 in Lactococcus lactis using single-particle tracking PALM.
Our open (single-molecule) microscopy project, the #miCube, is now on Github [link]! The page shows a detailed and updated overview of the components, some information on phasor-based SMLM, and many links to similar open hardware projects.
Meike joined the group for her BSc thesis and will work on DNA polymerase beta. Carel successfully defended his PhD thesis in March and decided to continue working on DNA polymerase beta by joining the Sweasy lab at Yale as a postdoctoral research assistent. All the best and we hope receiving new stocks soon!
…who joined the group for an 6 week internship (Andreas) and a short capita selecta (Hans). Andreas will try unifying some fragmented software packages for in vivo single particle tracking. Hans will push the limits of our ultrafast pSMLM algorithm for super-resolution microscopy.
Good news! The proposal “LocalBioFood: Localisation of bio-macromolecules in food matrices” submitted by Ilja Voets (TU/e) and our lab has been granted by the NWO innovation fund for chemistry [link].
Within the project, the three appointed PhD students will image different components in food, like proteins and carbohydrates, on a sub 100nm length scale using advanced fluorescence microscopy. The project is a collaboration between Eindhoven University of Technology, Wageningen University, Confocal.nl, Unilever and DSM.
…who joins the group for his MSc thesis. In collaboration with Dr. Klaus Mathwig (Pharmacy, Groningen), Dr. Peter van Baarlen (Host-Microbe Interactions, Wageningen) and Simon van der Els (NIZO food research and HMI, Wageningen), he will work towards monitoring bacterial conjugation in real time and at the single-cell level.
Good news! The proposal “Lipid Oxidation Control in Food Emulsions Enabled by Natural Strategies” submitted by Harry Gruppen (WUR) has been awarded a CHIPP grant by The Innovation Fund for Chemistry [link].
Our lab will head the work package titled “Localising oxidation: interfaces and transport” and a PhD position will be available in 2018.
Good news! Our proposal “Towards visualizing gene transfer at the single cell level using microfluidic devices” has been granted by the Innovation Program Microbiology, an initiative from the Laboratory of Microbiology at Wageningen University and Research.
In collaboration with Dr. Klaus Mathwig (Pharmacy, Groningen), Dr. Peter van Baarlen (Host-Microbe Interactions, Wageningen) and Simon van der Els (NIZO food research and HMI, Wageningen), we aim to monitor bacterial conjugation, in which DNA is transferred from a donor cell to a recipient cell either via conjugative plasmids or via integrative conjugative elements, in real time and at the single-cell level.
Hurrah, we finalised the design of our miCube (V0.1)! Details can be found [here] and include part numbers, CAD-drawings and STL files for CNC machining/milling or 3D printing published under a Creative Common license. Big shout out to Sander Baas and Koen Martens. If you have any questions, remarks or ideas, please feel to contact me.
Hohlbein lab - Wageningen 