Current snapshot of our constantly evolving miCube. For other related blog posts see [link]. The project has been supported by Wageningen Shared Research Facilities [link]. Latest updates: 2017-06-15 (3D printing settings); 2017-06-12 (STL files updated)
Exploded view with individual parts:
Excitation: Omicron Lighthub 6 (e.g. 405 nm, 488 nm, 561 nm, 640 nm laser) matched with suitable filters and polychroic beam splitters from Chroma. Settings for 3D printing: [link].
- Glass plate insert (size: 96-wells plate holder, 127.8 mm x 85.5 mm)
- ASI-Piezoconcept XYZ stage (ASI, Optophase)
- Objective (Nikon TIRF NA 1.49 HP SR, link)
- Top cover (see details below)
- Main block miCube (see details below)
- Dichroic mirror holder (Thorlabs: DFM1/M)
- Connector for dichroic mirror and mirror (Thorlabs: C4W-CC)
- 45˚ elliptical mirror (Thorlabs: KCB1E/M and BBE1-E02)
- Front cover miCube (see details below)
- Connector for excitation cover (Thorlabs: LCP02/M)
- Excitation cover (Thorlabs: SM1L20)
- TIRF lens (200 mm focal length, Thorlabs: AC254-200-A-ML)
- Spacer for excitation pathway (Thorlabs: SM1L05)
- Aperture (Thorlabs: SM1D12SZ)
- Reflective collimator (Thorlabs: RC12SMA-P01)
- Reflective collimator holder (see below)
- Right-angle mounting plate (Thorlabs: AP90/M)
- 25 mm Translation Stage (Thorlabs: PT1/M)
- Camera (Andor Zyla 4.2+, link)
- Camera mount (see details below)
- Astigmatism lens holder (see details below; lens: Thorlabs: LJ1516RM or LJ1144RM)
- same as above
- Astigmatism block (see details below)
- Emission cover (cut to length: Thorlabs: SC600)
- Swappable emission filters (Thorlabs: LCFH1-B and LCFH1-F)
- Tube filter block adapter (see details below)
- Filter housing (Thorlabs: LCFH1/M)
- Tube lens holder (Thorlabs: LCP01/M)
- Tube lens adapter (Thorlabs: SM2A20)
- Tube lens (200 mm, Thorlabs: ITL200 or similar)
| Milled aluminium parts for main Cube. [PDF-CAD-file ] | |
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(1) Stage insert (cover glass holder) [stl-file] |
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(4) Top cover miCube [stl-file] |
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(5) Main block miCube [stl-file] |
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(9) Front cover miCube [stl-file] |
| 3D printed parts for excitation path | |
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(16) Reflective collimator holder [stl-file] |
| 3D printed parts for detection path | |
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(20) Camera mount [stl-file] |
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(21,22) Astigmatism block [stl-file] |
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(23) Astigmatism lens holder [stl-file] |
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(26) Tube to filter-block connector [stl-file] |
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
