Category Archives: published

Published: High-throughput, non-equilibrium studies of single biomolecules using glass made nanofluidic devices

M. Fontana, C. Fijen, S. G. Lemay, K. Mathwig and J. Hohlbein, Lab on a Chip, 19, 79, 2019. [link] Single-molecule detection schemes offer powerful means to overcome static and dynamic heterogeneity inherent to complex samples. However, probing biomolecular interactions and reactions … Continue reading

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Published: Phasor based single-molecule localization microscopy in 3D (pSMLM-3D): an algorithm for MHz localization rates using standard CPUs

K.J.A. Martens, A.N. Bader, S. Baas, B. Rieger, J. Hohlbein, The Journal of Chemical Physics, 148, 123311, 2018, [link] We present a fast and model-free 2D and 3D single-molecule localization algorithm that allows more than 3 million localizations per second on … Continue reading

Posted in open access, published

Publication: A single-molecule FRET sensor for monitoring DNA synthesis in real time

C. Fijen, A. Montón Silva, A. Hochkoeppler and J. Hohlbein, Physical Chemistry Chemical Physics, 19, 4222-4230, 2017, [link] We developed a versatile DNA assay and framework for monitoring polymerization of DNA in real time and at the single-molecule level. The assay consists … Continue reading

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Publication: Visualization of BRI1 and SERK3/BAK1 Nanoclusters in Arabidopsis Roots

J. Hohlbein and A.N. Kapanidis, Methods in Enzymology: Single-molecule Enzymology Part A & B, published online, 2016, [link] Monitoring conformational changes in DNA polymerases using single-molecule Förster resonance energy transfer (smFRET) has provided new tools for studying fidelity-related mechanisms that promote the rejection of incorrect nucleotides before DNA synthesis. In addition to the previously known open and the […]

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Posted in open access, published

Publication: Fluorescence resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers

E. Ploetz, E. Lerner, F. Husada, M. Roelfs, S. Chung, J. Hohlbein, S. Weiss, T. Cordes, Scientific Reports, 6, 33257 , 2016, [link] Advanced microscopy methods allow obtaining information on (dynamic) conformational changes in biomolecules via measuring a single molecular distance … Continue reading

Posted in open access, published

Publication: A Quantitative Theoretical Framework For PIFE-FRET

E. Lerner, E. Ploetz, J. Hohlbein, T. Cordes, S. Weiss, The Journal of Physical Chemistry B, 120, 6401–6410, 2016, [link] Single molecule protein induced fluorescence enhancement (PIFE) serves as a molecular ruler at molecular distances inaccessible to other spectroscopic rulers such as … Continue reading

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Publication: Single molecule 3D orientation in Time and Space: A 6D dynamic study on fluorescent labeled lipid membranes.

R. Börner, N. Ehrlich, J. Hohlbein, C.G. Hübner, Journal of Fluorescence, 26, 963-975, 2016 [link] Interactions between single molecules profoundly depend on their mutual three-dimensional orientation to each other. Recently, we demonstrated a technique that allows the orientation determination of … Continue reading

Posted in accepted, published