Category Archives: published

Publication: A single-molecule FRET sensor for monitoring DNA synthesis in real time

C. Fijen, A. Montón Silva, A. Hochkoeppler and J. Hohlbein, Physical Chemistry Chemical Physics, 19, 4222-4230, 2017, [link] We developed a versatile DNA assay and framework for monitoring polymerization of DNA in real time and at the single-molecule level. The assay consists … Continue reading

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Publication: Visualization of BRI1 and SERK3/BAK1 Nanoclusters in Arabidopsis Roots

J. Hohlbein and A.N. Kapanidis, Methods in Enzymology: Single-molecule Enzymology Part A & B, published online, 2016, [link] Monitoring conformational changes in DNA polymerases using single-molecule Förster resonance energy transfer (smFRET) has provided new tools for studying fidelity-related mechanisms that promote the rejection of incorrect nucleotides before DNA synthesis. In addition to the previously known open and the […]

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Posted in open access, published

Publication: Fluorescence resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers

E. Ploetz, E. Lerner, F. Husada, M. Roelfs, S. Chung, J. Hohlbein, S. Weiss, T. Cordes, Scientific Reports, 6, 33257 , 2016, [link] Advanced microscopy methods allow obtaining information on (dynamic) conformational changes in biomolecules via measuring a single molecular distance … Continue reading

Posted in open access, published

Publication: A Quantitative Theoretical Framework For PIFE-FRET

E. Lerner, E. Ploetz, J. Hohlbein, T. Cordes, S. Weiss, The Journal of Physical Chemistry B, 120, 6401–6410, 2016, [link] Single molecule protein induced fluorescence enhancement (PIFE) serves as a molecular ruler at molecular distances inaccessible to other spectroscopic rulers such as … Continue reading

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Publication: Single molecule 3D orientation in Time and Space: A 6D dynamic study on fluorescent labeled lipid membranes.

R. Börner, N. Ehrlich, J. Hohlbein, C.G. Hübner, Journal of Fluorescence, 26, 963-975, 2016 [link] Interactions between single molecules profoundly depend on their mutual three-dimensional orientation to each other. Recently, we demonstrated a technique that allows the orientation determination of … Continue reading

Posted in accepted, published

Publication: Complex coacervate core micelles with spectroscopic labels for diffusometric probing of biopolymer networks

N. Bourouina, D. de Kort, F. Hoeben, H. Janssen, H. Van As, J. Hohlbein, J. van Duynhoven, J.M. Kleijn, Langmuir, 31, 12635-12643, 2015, [link] We present the design, preparation and characterization of two types of complex coacervate core micelles (C3Ms) with … Continue reading

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Publication: New technologies for DNA analysis – a review of the READNA Project

S. McGinn, D. Bauer, T. Brefort, L. Dong, A. El-Sagheer, A. Elsharawy, G. Evans, E. Falk-Sörqvist, M. Forster, S. Fredriksson, P. Freeman, C. Freitag, J. Fritzsche, S. Gibson, M. Gullberg, M. Gut, S. Heath, I. Heath-Brun, A.J. Heron, J. Hohlbein, … Continue reading

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