Category Archives: published

Publication: Single molecule 3D orientation in Time and Space: A 6D dynamic study on fluorescent labeled lipid membranes.

R. Börner, N. Ehrlich, J. Hohlbein, C.G. Hübner, Journal of Fluorescence, 26, 963-975, 2016 [link] Interactions between single molecules profoundly depend on their mutual three-dimensional orientation to each other. Recently, we demonstrated a technique that allows the orientation determination of … Continue reading

Posted in accepted, published

Publication: Complex coacervate core micelles with spectroscopic labels for diffusometric probing of biopolymer networks

N. Bourouina, D. de Kort, F. Hoeben, H. Janssen, H. Van As, J. Hohlbein, J. van Duynhoven, J.M. Kleijn, Langmuir, 31, 12635-12643, 2015, [link] We present the design, preparation and characterization of two types of complex coacervate core micelles (C3Ms) with … Continue reading

Posted in published

Publication: New technologies for DNA analysis – a review of the READNA Project

S. McGinn, D. Bauer, T. Brefort, L. Dong, A. El-Sagheer, A. Elsharawy, G. Evans, E. Falk-Sörqvist, M. Forster, S. Fredriksson, P. Freeman, C. Freitag, J. Fritzsche, S. Gibson, M. Gullberg, M. Gut, S. Heath, I. Heath-Brun, A.J. Heron, J. Hohlbein, … Continue reading

Posted in published

Publication: Camera-based single-molecule FRET detection with improved time resolution

S. Farooq and J. Hohlbein, Physical Chemistry Chemical Physics, 17, 27862, 2015, [link], open access The achievable time resolution of camera-based single-molecule detection is often limited by the frame rate of the camera. Especially in experiments utilizing single-molecule Förster resonance … Continue reading

Posted in open access, published

Publication: Real-time single-molecule studies of the motions of DNA polymerase fingers illuminate DNA synthesis mechanisms

G.W. Evans, J. Hohlbein, T. Craggs, L. Aigrain and A.N. Kapanidis, Nucleic Acids Research, 43, 5998-6008, 2015, [link], open access DNA polymerases maintain genomic integrity by copying DNA with high fidelity. A conformational change important for fidelity is the motion of … Continue reading

Posted in open access, published

Publication: Studying DNA-protein interactions with single-molecule Förster resonance energy transfer

S. Farooq, C. Fijen, J. Hohlbein, Protoplasma, SPECIAL ISSUE: NEW/EMERGING TECHNIQUES IN BIOLOGICAL MICROSCOPY,  251, 317-332, 2014 [link] Single-molecule Förster resonance energy transfer (smFRET) has emerged as a powerful tool for elucidating biological structure and mechanisms on the molecular level. Here, we … Continue reading

Posted in published

Publication: A Novel Parallel Nanomixer for High-Throughput Single-Molecule Fluorescence Detection

K. Mathwig, S. Schlautmann, S. G. Lemay, J. Hohlbein, A Novel Parallel Nanomixer for High-Throughput Single-Molecule Fluorescence Detection, Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Science, Freiburg, Germany, Oct. 27 – 31 (2013) 1385,  [link] This … Continue reading

Posted in published