Category Archives: published

Publication: Alternating-laser excitation: single-molecule FRET and beyond

J. Hohlbein, T.D. Craggs, T. Cordes, Chemical Society Reviews, 43, 1156-1171, 2014, [link] The alternating-laser excitation (ALEX) scheme continues to expand the possibilities of fluorescence-based assays to study biological entities and interactions. Especially the combination of ALEX and single-molecule Förster … Continue reading

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Publication: Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion (open access)

J. Hohlbein, L. Aigrain, T.D. Craggs, O. Bermek, O. Potapova, P. Shoolizadeh, N.D.F. Grindley, C.M. Joyce, A.N. Kapanidis, Nature Communications, 4,  2131, 2013, [link], open access The fidelity of DNA polymerases depends on conformational changes that promote the rejection of incorrect nucleotides … Continue reading

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Publication: Characterization of Dark Quencher Chromophores as Nonfluorescent Acceptors for Single-Molecule FRET

L. Le Reste, J. Hohlbein, K. Gryte, A.N. Kapanidis, Biophysical Journal, 102, 11, 2658-2668, 2012, [link] Dark quenchers are chromophores that primarily relax from the excited state to the ground state nonradiatively (i.e., are dark). As a result, they can serve … Continue reading

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Publication: Identifying molecular dynamics in single-molecule FRET experiments with Burst Variance Analysis

J.P. Torella, S.J. Holden, Y. Santoso, J. Hohlbein, A.N. Kapanidis, Biophysical Journal, Vol. 100, Issue 6, 1568-1577, 2011, [link] Histograms of single-molecule Förster resonance energy transfer (FRET) efficiency are often used to study the structures of biomolecules and relate these … Continue reading

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Publication: Defining the limits of single-molecule FRET resolution in TIRF microscopy

S.J. Holden, S. Uphoff, J. Hohlbein, D. Yadin, L. Le Reste, O.J. Britton, A.N. Kapanidis, Biophysical Journal, Vol. 99, Issue 9, 2011, 3102-3111 [link] Single-molecule FRET (smFRET) has long been used as a molecular ruler for the study of biology … Continue reading

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Publication: Surfing on a new wave of single-molecule methods

J. Hohlbein, K. Gryte, M. Heilemann, A.N. Kapanidis, Phys. Biol., 7, 031001, 2010, [link] Single-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule toolbox. In this review, we discuss recent advances in fluorescence instrumentation and … Continue reading

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Publication: Conformational transitions in DNA polymerase I revealed by single-molecule FRET

Y. Santoso, C.M. Joyce, O. Potapova, L. Le Reste, J. Hohlbein, J.P. Torella, N.D.F. Grindley, and A.N. Kapanidis, PNAS, 107, 715, 2010, [link] The remarkable fidelity of most DNA polymerases depends on a series of early steps in the reaction … Continue reading

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