Good news! Our proposal “Nanofluidic devices for high-throughput single-molecule fluorescence detection (Nanofluidic-SMFD)” has been granted by the European Commission (FP7-PEOPLE-2013-CIG, #630992). The proposed work aims at utilising nanofluidic devices to overcome current limitations in single-molecule-fluorescence detection. Specifically, we are hoping to monitor enzymatic reactions such as DNA polymerisation in real time without immobilising any of the involved molecules to a surface as it is currently necessary. For this project, we will continue our collaboration with Klaus Mathwig (www.kmathwig.com).
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- Pre-print: Direct visualization of native CRISPR target search in live bacteria reveals Cascade DNA surveillance mechanism
- Published: Evaluating single-particle tracking by photo-activation localization microscopy (sptPALM) in Lactococcus lactis
- News: Welcome to Suyeon Yang and George Vogelaar
- Published: High-throughput, non-equilibrium studies of single biomolecules using glass made nanofluidic devices
- Pre-print: An open microscopy framework suited for tracking dCas9 in live bacteria